ORIGINAL PAPER
The effect of increasing amounts of synovial fluid isolated from patients with rheumatoid arthritis on the respiratory burst of human neutrophils
Online publication date: 2009-12-29
Reumatologia 2009;47(5):273-281
KEYWORDS
ABSTRACT
It has been proposed that the inappropriate release of oxidants and proteolytic enzymes from activated neutrophils is responsible for joint damage observed in rheumatoid arthritis (RA). The present study examines the effect of increasing concentrations of synovial fluid [SF] (0-80%) on the oxidative response of neutrophils isolated from SF of RA patients and the blood of healthy subjects. To reflect better the real conditions of the inflamed joint we used in our experiments higher concentrations of SF than was previously reported. We found that increasing the concentrations of SF has a different effect on lucigenin-dependent and luminol-dependent CL response.
REFERENCES (20)
1.
Allen RC. Halide dependence of the myeloperoxidase-mediated antimicrobial system of the polymorphonuclear leukocyte in the phenomenon of electronic excitation. Biochem Biophys Res Commun 1975; 63: 675-683.
2.
Nurcombe HL, Edwards SW. Role of myeloperoxidase in intracellular and extracellular chemiluminescene of neutro-phils. Ann Rheum Dis 1989; 48: 56-62.
3.
Bender JG, Van Epps DE, Searles R, et al. Altered functions of synovial fluid granulocytes in patients with acute inflammatory arthritis: evidence for activation of neutrophils and its mediation by a factor present in synovial fluid. Inflammation 1986; 10: 443-453.
4.
Gale R, Bertouch JV, Bradley J, et al. Direct activation of neutrophil chemiluminescence by rheumatoid sera and synovial fluid. Ann Rheum Dis 1983; 42: 158-162.
5.
Robinson J, Watson F, Bucknall RC, et al. Activation of neutrophil reactive-oxidant production by synovial fluid from patients with inflammatory joint disease. Biochem J 1992; 286: 345-351.
6.
Duluray B, Badesha JS, Dieppe PA, et al. Oxidative response of polymorphonuclear leucocytes to synovial fluid from patients with rheumatoid arthritis. Ann Rheum Dis 1990; 49: 661-664.
7.
Nurcombe HL, Bucknall RC, Edwards SW. Activation of the neutrophil myeloperoxidase H2O2 system by synovial fluid isolated from patients with rheumatoid arthritis. Ann Rheum Dis 1991; 50: 237-242.
8.
Duluray B, Yea CM, Elson CJ. Inhibition of myeloperoxidase by synovial fluid and serum, Ann. Rheum Dis 1991; 50: 383-388.
9.
Bostan M, Brasoveanu LI, Livescu A, et al. Effect of synovial fluid on the respiratory burst of granulocytes in rheumatoid arthritis. J Cell Mol Med 2001; 5: 188-194.
10.
Robinson JJ, Watson F, Phelan M, et al. Activation of neutrophils by soluble and insoluble immunoglobulin aggregates from synovial fluid of patients with rheumatoid arthritis. Ann Rheum Dis 1993; 52: 347-353.
11.
Edwards SW, Hallett MB. Seeing the wood for the trees: the forgoten role of neutrophils in rheumatoid arthritis. Immunol Today 1997; 18: 320-324.
12.
Davies EV, Williams BD, Campbell AK. Synovial fluid polymorphonuclear leukocytes from patients with rheumatoid arthritis have reduced MPO and NADPH-oxidase activity. Brit J Rheum 1990; 29: 415-421.
13.
Sato H, Takahashi T, Ide H, et al. Antioxidant activity of synovial fluid, hyaluronic acid, and two subcomponents of hyaluronic acid. Arthritis Rheum 1988; 31: 63-81.
14.
Nurcombe HL, Bucknall RC, Edwards SW. Neutrophils isolated from the synovial fluid of patients with rheumatoid arthritis: priming and activation in vitro. Ann Rheum Dis 1991; 50: 147-153.
15.
Eggleton P, Wang L, Penhallow J. Differences in oxidative response of subpopulations of neutrophils from healthy subjects and patients with rheumatoid arthritis. Ann Rheum Dis 1995; 54: 916-923.
16.
Fossati G, Bucknall RC, Edwards SW. Insoluble and soluble immune complexes activate neutrophils by distinct activation mechanisms: changes in functional responses induced by priming with cytokines. Ann Rheum Dis 2002; 61: 13-19.
17.
Sugisawa H, Itou T, Ichimura Y, et al. Bovine milk enhances the oxidative burst activity of polymorphonuclear leukocytes in low concentration. J Vet Med Sci 2002; 64: 113-116.
18.
Bell AL, Magill MK, Irvine AE. Human blood and synovial fluid neutrophils cultured in vitro undergo programmed cell death which is promoted by the addition of synovial fluid. Ann Rheum Dis 1995; 54: 910-915.
19.
Savill J. Apoptosis in resolution of inflammation. J Leuko Biol 1997; 61: 375-80.
20.
Iking-Konert C, Ostendorf B, Sander O, et al. Transdif-ferentiation of polymorphonuclear neutrophils to dendritic-like cells at the site of inflammation in rheumatoid arthritis: evidence for activation by the T cells. Ann Rheum Dis 2005; 64: 1436-1442.
Copyright: © Narodowy Instytut Geriatrii, Reumatologii i Rehabilitacji w Warszawie. This is an Open Access journal, all articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (https://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
| eISSN: | 2084-9834 |
| ISSN: | 0034-6233 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
