ORIGINAL PAPER
The effects of β-glucan isolated from Pleurotus ostreatus on the development of arthritis and methotrexate treatment in rats with adjuvant arthritis
Online publication date: 2013-09-11
Reumatologia 2013;51(4):277-283
KEYWORDS
ABSTRACT
Aim of the study: The purpose of this study was to evaluate the effect of β-(1,3/1,6)-D-glucan isolated from mushroom Pleurotus ostreatus (β-glucan-PO) on the preventive treatment and therapy of adjuvant arthritis (AA) and methotrexate (MTX) treatment in rats.
Material and methods: Groups of rats with AA were preventively treated with methotrexate (1 mg/kg/week), β-glucan-PO (1 mg/kg every second day) or their combination for a period of 28 days from adjuvant application. Methotrexate and β-glucan-PO (15 mg/kg every day) were also applied therapeutically from day 13 to day 35 after immunization. Body mass, hind paw swelling, arthrogram scores, and the level of serum albumin were measured as markers of inflammation and arthritis.
Results: Preventive treatment with a low dose of MTX significantly inhibited the markers of both inflammation and arthritis. Methotrexate and its combination with β-glucan-PO significantly increased the body mass of arthritic rats. β-glucan-PO administered alone had no effect on body mass but significantly decreased both the hind paw swelling and arthritic score. In combination with MTX, β-glucan-PO markedly potentiated the beneficial effects of MTX, which resulted in a more significant reduction of hind paw swelling and arthritic scores. The concentration of albumin in the serum of arthritic controls was significantly lower than in the healthy controls. Both MTX alone and the combination treatment with MTX + β-glucan-PO significantly inhibited the decrease of serum albumin. Therapeutically, MTX had no significant effect on the markers of inflammation and arthritis. β-glucan-PO alone in a high dose (15 mg/kg body mass) inhibited hand paw swelling, arthrogram scores, and the decrease of serum albumin.
Conclusions: β-glucan-PO alone positively affected both the development and treatment of adjuvant arthritis in rats and increased the efficacy of basal treatment with MTX. In patients with rheumatoid arthritis this immunomodulator may prevent secondary infections and restore impaired immunological homeostasis.
Material and methods: Groups of rats with AA were preventively treated with methotrexate (1 mg/kg/week), β-glucan-PO (1 mg/kg every second day) or their combination for a period of 28 days from adjuvant application. Methotrexate and β-glucan-PO (15 mg/kg every day) were also applied therapeutically from day 13 to day 35 after immunization. Body mass, hind paw swelling, arthrogram scores, and the level of serum albumin were measured as markers of inflammation and arthritis.
Results: Preventive treatment with a low dose of MTX significantly inhibited the markers of both inflammation and arthritis. Methotrexate and its combination with β-glucan-PO significantly increased the body mass of arthritic rats. β-glucan-PO administered alone had no effect on body mass but significantly decreased both the hind paw swelling and arthritic score. In combination with MTX, β-glucan-PO markedly potentiated the beneficial effects of MTX, which resulted in a more significant reduction of hind paw swelling and arthritic scores. The concentration of albumin in the serum of arthritic controls was significantly lower than in the healthy controls. Both MTX alone and the combination treatment with MTX + β-glucan-PO significantly inhibited the decrease of serum albumin. Therapeutically, MTX had no significant effect on the markers of inflammation and arthritis. β-glucan-PO alone in a high dose (15 mg/kg body mass) inhibited hand paw swelling, arthrogram scores, and the decrease of serum albumin.
Conclusions: β-glucan-PO alone positively affected both the development and treatment of adjuvant arthritis in rats and increased the efficacy of basal treatment with MTX. In patients with rheumatoid arthritis this immunomodulator may prevent secondary infections and restore impaired immunological homeostasis.
REFERENCES (26)
1.
Akramiené D, Kondratos A, Didžiapetriené J, Kévelaitis E. Effects of β-glucans on the immune system. Medicina (Kaunas) 2007; 43: 597-606.
2.
Muta T. Molecular basis for invertebrate innate immune recognition of (13)-beta-D-glucan as a pathogen-associated molecular pattern. Curr Pharm Des 2006; 12: 4155-4161.
3.
Ross G, Cain J, Myones B, et al. Specificity of membrane complement receptor type 3 (CR3) for β-glucans. Complement 1987; 4: 61-74.
4.
Brown GD, Tailor PR, Reid DM, et al. Dectin-1 is a major β-glucan receptor on macrophages. J Exp Med 2002; 196: 407-412.
5.
Brown GD, Gordon S. Immune recognition of fungal beta-glucans. Cell Microbiol 2005; 7: 471-479.
6.
Ben-Ami R, Lewis RE, Kontoyiannis DP. Immunocompromised hosts: immunopharmacology of modern antifungals. Clin Infect Dis 2008; 47: 226-235.
7.
Kernodle DS, Gates H, Kaiser AB. Prophylactic anti-infective activity of poly-[1-6]-beta-D-glucopyranosyl-[1-3]-beta-D-glucopryanose glucan in a Guinea pig model of staphylococcal wound infection. Antimicrob Agents Chemother 1998; 42: 545-549.
8.
Větvička V, Vashishta A, Saraswat-Ohri S, Vetvickova J. Immunological effects of yeast- and mushroom-derived beta-glucans. J Med Food 2008; 11: 615-622.
9.
Weitberg AB. A phase I/II trial of beta-(1,3)/(1,6) D-glucan in the treatment of patients with advanced malignancies receiving chemotherapy. J Exp Clin Cancer Res 2008; 27: 40.
10.
Mantovani MS, Bellini MF, Angeli JP, et al. Beta-Glucans in promoting health: prevention against mutation and cancer. Mutat Res 2008; 658: 154-161. .
11.
Bobek P, Galbavy S. Effect of pleuran (beta-glucan from Pleurotus ostreatus) on the antioxidant status of the organism and on dimethylhydrazine-induced precancerous lesions in rat colon. Br J Biomed Sci 2001; 58: 164-168.
12.
Bobek P, Galbavy S, Ozdin L. Effect of oyster mushroom (Pleurotus ostreatus) on pathological changes in dimethylhydrazine-induced rat colon cancer. Oncol Rep 1998; 5: 727-730.
13.
Nosál'ová V, Bobek P, Černá S, et al. Effects of pleuran (beta-glucan isolated from Pleurotus ostreatus) on experimental colitis in rats. Physiol Res 2001; 50: 575-581.
14.
Smiderle FR, Olsen LM, Carbonero ER, et al. Anti-inflammatory and analgesic properties in a rodent model of a (13),(16)-linked beta-glucan isolated from Pleurotus pulmonarius. Eur J Pharmacol 2008; 597: 86-91.
15.
Bauerová K, Paulovičová E, Mihalová D, et al. Study of new ways of supplementary and combinatory therapy of rheumatoid arthritis with immunomodulators Glucomannan and Imunoglukán® in adjuvant arthritis. Toxicol Industrial Health 2009; 25: 329-335.
16.
Stančíková M, Rovenský J, Švík K, et al. Effect of immunostimulators on adjuvant arthritis in rats (in Slovak). Rheumatologia 2008; 22: 9-11.
17.
Kogan G, Staško A, Bauerová K, et al. Antioxidant properties of yeast (13)-β-d-glucan studied by electron paramagnetic resonance spectroscopy and its activity in the adjuvant arthritis. Carbohydrate Polymers (Elsevier) 2005; 61: 18–28. .
18.
Sener G, Ekşioglu-Demiralp E, Cetiner M, et al. Beta-glucan ameliorates methotrexate-induced oxidative organ injury via its antioxidant and immunomodulatory effects. Eur J Pharmacol 2006; 542: 170-178.
20.
Welles WL, Silkworth J, Oronsky AL, et al. Studies on the effect of low dose methotrexate in adjuvant arthritis. J Rheumatol 1985; 12: 904-906. .
21.
Connolly KM, Stecher VJ, Danis E, et al. Alteration of interleukin-1 production and the acute phase response following medication of adjuvant arthritic rats with cyclosporin-A or methotrexate. Int J Immunopharmacol 1988; 10: 717-728. .
22.
Cleary JA, Kelly GE, Husband AJ. The effect of molecular weight and beta-1,6-linkages on priming of macrophage function in mice by (1,3)-beta-D glucan. Immunol Cell Biol 1999; 77: 395-403.
23.
Onderdonk AB, Cisneros RL, Hinkson P, Ostroff G. Anti-infective effect of poly-beta-1-6-glucotriosyl-beta1-3-glucopyranose glucan in vivo. Infect Immun 1992; 60: 1642-1647.
24.
Bedirli A, Kerem M, Pasaoglu H, et al. Beta-glucan attenuates inflammatory cytokine release and prevents acute lung injury in an experimental model of sepsis. Shock 2007; 27: 397-401.
25.
Hetland G, Lovik M, Wiker HG. Protective effect of beta-glucan against mycobacterium bovis, BCG infection in BALB/c mice. Scand J Immunol 1998; 47: 548-553. .
26.
Hetland G, Sandven P. Beta-1,3-glucan reduces growth of Mycobacterium tuberculosis in macrophage cultures. FEMS Immunol Med Microbiol 2002; 33: 41-45.
Copyright: © Narodowy Instytut Geriatrii, Reumatologii i Rehabilitacji w Warszawie. This is an Open Access journal, all articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (https://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
Share
RELATED ARTICLE
| eISSN: | 2084-9834 |
| ISSN: | 0034-6233 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
