Application of fluorescence spectroscopy in the amyloidosis diagnosis: a preliminary report
More details
Hide details
Online publication date: 2010-09-21
Reumatologia 2010;48(4):230-236
The aim of this study was to evaluate the usefulness of fluorescence spectroscopy studies in the diagnosis of secondary AA amyloidosis. Histopathological, immunohistochemical and fluorescence spectroscopy evaluations of abdominal fat tissue specimens of 12 patients with rheumatoid arthritis (RA) and secondary amyloidosis were performed. The control group comprised 8 RA patients with clinical suspicion of amyloidosis, unconfirmed in histopathological examination. In 9 cases, the analysis of autofluorescence spectra showed differences between the slices containing amyloid deposits and healthy tissue. A characteristic feature of tissue slices with amyloidosis was the appearance of a second maximum in the fluorescence spectrum chart. In 3 cases, the shape of the fluorescence spectra did not enable an unequivocal differential diagnosis. The histopathological tissue examination remains the gold standard in the diagnosis of amyloidosis. Preliminary results indicate that the fluorescence spectrum analysis can be a diagnosis support method, but to assess its usefulness the group of patients needs to be increased.
Picken MM. Amyloidosis-where are we now and where are we heading? Arch Pathol Lab Med 2010; 134: 545-551.  .
Perfetto F, Moggi-Pignone A, Livi R, et al. Systemic amyloidosis: a challenge for the rheumatologist. Nat Rev Rheumatol. 2010; 6: 417-429.  .
Fornalska L, Lewandowski J, Hrycaj P. Opis przypadku – współistnienie ciężkiego reumatoidalnego zapalenia stawów i amyloidozy. Reumatologia 2009; 47: 385-388.  .
Chemperek E, Majdan M, Kurowska M, i wsp. Opis przypadku – wole jako manifestacja kliniczna wtórnej amyloidozy w przebiegu reumatoidalnego zapalenia stawów. Reumatologia 2006; 44: 374-377.  .
Lachmann HJ, Goodman HJ, Gilbertson JA, et al. Natural history and outcome in systemic AA amyloidosis. N Engl J Med 2007; 356: 2361-2371.  .
Prochorec-Sobieszek M, Bilińska Z, Grzybowski J, et al. Assessment of the inflammatory process by endomyocardial biopsy in patients with dilated cardiomyopathy based on histopathological and immunohistochemical methods. Kardiol Pol 2006; 64: 479-487.  .
Thomas TP, Myaing MT, Ye JY, et al. Detection and analysis of tumor fluorescence using a two-photon optical fiber probe. Biophys J 2004; 86: 3959-3965.  .
Chwirot BW, Chwirot S, Sypniewska N, et al. Fluorescence in situ detection of human cutaneous melanoma: study of diagnostic parameters of the method. J Invest Dermatol 2001; 117: 1449-1451.  .
Szczęsny W, Żuchowski P, Fisz JJ, et al. The ultrastructure of rectus sheath in patients with inguinal hernias and healthy controls-an evaluation by fluorescent and microspectrometric techniques. Hernia 2009; 13 suppl. 1: 64-65. .
Jeka S, Żuchowski P, Fisz JJ, et al. Wykorzystanie zjawiska fluorescencji w diagnostyce chorób układowych tkanki łącz­nej. Pierwsze Krajowe Spotkanie Reumatologiczne, Łódź 9-10.10.2009. .
Kwan AC, Duff K, Gouras GK, et al. Optical visualization of Alzheimer’s pathology via multiphoton-excited intrinsic fluorescence and second harmonic generation. Opt Express 2009; 17: 3679-3689. .
Grateau G, Kyle RA, Skinner M. Amyloid and Amyloidosis. CRC Press, 2005. .
Naiki H, Higuchi K, Hosokawa M, i wsp. Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavine T. Anal Biochem 1989; 177: 244-249. .
Koh CJ, Lee M. Fluorescence lifetime imaging microscopy of amyloid aggregates. Bull Korean Chem Soc 2006; 27: 477-478. .
Ozawa D, Yagi H, Ban T, et al. Laser-induced propagation and destruction of amyloid {beta} fibrils. J Biol Chem 2010; 285: 19660-19667.
Copyright: © Narodowy Instytut Geriatrii, Reumatologii i Rehabilitacji w Warszawie. This is an Open Access journal, all articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (https://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
Journals System - logo
Scroll to top