ORIGINAL PAPER
Serum IgG galactosylation in psoriatic arthritis patients undergoing a biological treatment. A preliminary report
Submission date: 2014-06-11
Final revision date: 2014-11-18
Acceptance date: 2014-11-24
Online publication date: 2014-11-30
Publication date: 2014-12-31
Reumatologia 2014;52(6):369-376
KEYWORDS
TOPICS
Assessment of disease activity and quality of life in rheumatic diseasesDiagnostics and imaging in rheumatic diseases
ABSTRACT
Aim of the study: A group of psoriatic arthritis patients (PSA, 11 cases) has undergone a treatment. For each patient two serum samples were collected: before the treatment (sample before) and after the treatment (sample after). Performed investigation aimed to provide an information whether a treatment of the PSA patients improves the galactosylation of IgG N-glycans, which previously was shown for rheumatoid arthritis (RA) patients.
Material and methods: Immunoglobulin G (IgG) was isolated from serum of PSA patients, before and after the treatment, using affinity chromatography on Protein A-Sepharose, and was further analyzed regarding the galactose content, using gas chromatography-mass spectrometry (GC-MS) method and ELISA test, performed with two lectins: Ricinus communis (RCA-I) and Griffonia simplicifolia (GSL-II). Based on ELISA results an agalactosylation factor (AF) was calculated for each before and after IgG sample.
Results: Obtained data regarded galactose content in IgG, AF calculation and estimation of two laboratory indices of inflammation: erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) concentration in serum. Based on statistical methods it was shown that average difference (d) of after and before measures of AF for PSA patients was MedHL = –0.097 (95% CI: –0.49–0.25), which means that applied treatment did not affect AF value for this group of patients in a statistically significant manner (p = 0.2936).
Conclusions: The study demonstrated that, in the contrary to RA patients, IgG galactosylation in PSA patients, undergoing a treatment, showed no statistically significant changes.
Material and methods: Immunoglobulin G (IgG) was isolated from serum of PSA patients, before and after the treatment, using affinity chromatography on Protein A-Sepharose, and was further analyzed regarding the galactose content, using gas chromatography-mass spectrometry (GC-MS) method and ELISA test, performed with two lectins: Ricinus communis (RCA-I) and Griffonia simplicifolia (GSL-II). Based on ELISA results an agalactosylation factor (AF) was calculated for each before and after IgG sample.
Results: Obtained data regarded galactose content in IgG, AF calculation and estimation of two laboratory indices of inflammation: erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) concentration in serum. Based on statistical methods it was shown that average difference (d) of after and before measures of AF for PSA patients was MedHL = –0.097 (95% CI: –0.49–0.25), which means that applied treatment did not affect AF value for this group of patients in a statistically significant manner (p = 0.2936).
Conclusions: The study demonstrated that, in the contrary to RA patients, IgG galactosylation in PSA patients, undergoing a treatment, showed no statistically significant changes.
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Copyright: © Narodowy Instytut Geriatrii, Reumatologii i Rehabilitacji w Warszawie. This is an Open Access journal, all articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (https://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
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